muc2 polyclonal antibody  (Proteintech)

Journal: Experimental and Therapeutic Medicine

Article Title: Appendiceal mucinous tumour resulting in autoamputation of the appendix: A case report and literature review

doi: 10.3892/etm.2025.13043

Figure Lengend Snippet: Immunohistochemical markers in the removed appendix tissue. (A) CKpan positivity; (B) CK7 negativity; (C) CK20 positivity; (D) caudal type homeobox 2 positivity; (E) MUC2 positivity; (F) MUC5AC negativity; (G) PAX-2 negativity; (H) PAX-8 negativity; and (I) Ki-67 negativity. CK, cytokeratin; PAX, paired box; MUC, mucin.

Article Snippet: Primary antibody details were as follows: CK-pan mouse monoclonal antibody (7H8C4; 1:200; cat. no. EM1712-42; HUABIO), CK7 recombinant monoclonal antibody (1:2,000; cat. no. 86153-7-RR; Proteintech Group, Inc.), CK20 recombinant monoclonal antibody (1:4,000; cat. no. 82428-1-RR; Proteintech Group, Inc.), CDX2 recombinant monoclonal antibody (1:1,000; cat. no. 82659-1-RR; Proteintech Group, Inc.), MUC2 polyclonal antibody (1:2,000; cat. no. 27675-1-AP; Proteintech Group, Inc.), MUC5AC polyclonal antibody (1:500; cat. no. 20725-1-AP; Proteintech Group, Inc.), PAX2 polyclonal antibody (1:1,000; cat. no. 29307-1-AP; Proteintech Group, Inc.), PAX8 polyclonal antibody (1:800; cat. no. 10336-1-AP; Proteintech Group, Inc.) and Ki-67 polyclonal antibody (1:4,000; cat. no. 27309-1-AP; Proteintech Group, Inc.).

Techniques: Immunohistochemical staining

Journal: Animal Nutrition

Article Title: Terpine-4-ol alleviates lipopolysaccharide-induced intestinal injury in weaned piglets by regulating intestinal epithelial cell homeostasis via the Wnt/β-catenin signaling pathway

doi: 10.1016/j.aninu.2024.12.010

Figure Lengend Snippet: Effects of terpine-4-ol on the number of goblet cells, the protein expression of mucin 2 (Muc2) and chromogranin A (ChgA) in the small intestine of immune-stressed piglets. The goblet cells in the jejunum and ileum (A, B). The immunohistochemical staining of Muc2 in the jejunum and ileum (C). The immunohistochemical staining of ChgA in the jejunum and ileum (D). CON, control group; LPS, lipopolysaccharide challenged group; LTP, low dose of terpine-4-ol supplemented group; MTP, middle dose of terpine-4-ol supplemented group; HTP, high dose of terpine-4-ol supplemented group. Different small letters above data columns indicate significant differences among different groups ( P < 0.05).

Article Snippet: The sections were incubated with the first primary antibodies of antigen Ki-67 (Ki-67) polyclonal antibody (1:400, PA5-19462, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), chromogranin A antibody (1:2, ab53464, Abcam, Cambridge, UK), mucin 2 (Muc2) polyclonal antibody (1:1500, 27675-1-AP, Proteintech Group, Rosemont, IL, USA), villin polyclonal antibody (1:100, ab233155, Abcam, Cambridge, UK), cleaved caspase 3 antibody (1:2200, 9664S, Cell Signaling Technology, Danvers, MA, USA) for 1 h at room temperature, respectively.

Techniques: Expressing, Immunohistochemical staining, Staining, Control

Journal: Animal Nutrition

Article Title: Terpine-4-ol alleviates lipopolysaccharide-induced intestinal injury in weaned piglets by regulating intestinal epithelial cell homeostasis via the Wnt/β-catenin signaling pathway

doi: 10.1016/j.aninu.2024.12.010

Figure Lengend Snippet: Effects of terpine-4-ol on the number of goblet cells, the protein expression of mucin 2 (Muc2) and chromogranin A (ChgA) in the small intestine of immune-stressed piglets. The goblet cells in the jejunum and ileum (A, B). The immunohistochemical staining of Muc2 in the jejunum and ileum (C). The immunohistochemical staining of ChgA in the jejunum and ileum (D). CON, control group; LPS, lipopolysaccharide challenged group; LTP, low dose of terpine-4-ol supplemented group; MTP, middle dose of terpine-4-ol supplemented group; HTP, high dose of terpine-4-ol supplemented group. Different small letters above data columns indicate significant differences among different groups ( P < 0.05).

Article Snippet: The sections were incubated with the first primary antibodies of antigen Ki-67 (Ki-67) polyclonal antibody (1:400, PA5-19462, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), chromogranin A antibody (1:2, ab53464, Abcam, Cambridge, UK), mucin 2 (Muc2) polyclonal antibody (1:1500, 27675-1-AP, Proteintech Group, Rosemont, IL, USA), villin polyclonal antibody (1:100, ab233155, Abcam, Cambridge, UK), cleaved caspase 3 antibody (1:2200, 9664S, Cell Signaling Technology, Danvers, MA, USA) for 1 h at room temperature, respectively.

Techniques: Expressing, Immunohistochemical staining, Staining, Control